Anthurium , belonging to theAraceaefamily is a crop of commercial-grade grandness in India and many other countries . It is cultivated for its beautiful cut flowers , cut foliage and as a potted industrial plant . In 2016 , tail-flower grown in nursery on the RHREC campus , GKVK Bengaluru , Karnataka , India , establish various types of disease symptom with an initial incidence of some 25 % . In 2018 , the disease incidence had increased to 80 % along with heavy plague of thrips . Plants showed chlorotic and necrotic lesions on spathe ( Fig . 1 ) and leaves ( Fig . 2 ) , and miscreation of spathes and necrotic streaks on flower stalks . These symptom were observed on anthurium cvs . Acropolish , Cheers , Fire , Tropical and Xavia . The symptoms resemble those caused byTomato recognize wilt viruson anthurium report from Hawaii ( Uchidaet al . , 1999 ) .
pattern 1 : Symptoms of Groundnut bud gangrene computer virus infection on anthurium spathes in cultivar : a ) Xavia , b ) Tropical , c ) Fire and d ) Acropolish .
Figure 2 : symptom of Groundnut bud necrosis virus infection on anthurium leaves : a ) greensick wound , and b ) necrotic lesions .
Twenty samples comprising either leaf or spathe were collected indiscriminately from symptomatic plant and tested along with buffer and healthy plant controls for the presence of serogroup IV tospoviruses using polyclonal antibodies put up againstGroundnut bud necrosisvirus ( GBNV ) ( reported to discover all serogroup IV orthotospoviruses ( Mandal & Jain , 2010 ) ) by DAC - ELISA . ELISA results were positive for all symptomatic plants with mean absorbance values ( A405 ) ranging from 0.65 to 1.4 liken to 0.12 in the healthy plant control . To confirm the comportment of an orthotospovirus metal money , total RNA was excerpt from symptomatic and healthy leaf sampling using the SpectrumTMPlant entire RNA Kit ( Sigma - Aldrich USA ) and subjected to RT - PCR analysis . The serogroup IV orthotospovirus group - specific rearward primer ( 5′-AGAGCAATCGAGGCGCTAATAA-3′ ) and single species - specific forward primers designed using successiveness analysis ( bang or CLUSTAL O ( 1.2.4 ) and Oligo Analyzer ( 1.0.2 ) were used for RT - PCR . An amplicon of the expected size ( 898 bp ) was obtained with the GBNV - specific forward-moving primer ( 5′-TTACAATTCCAGCGAAGGAC-3′ ) from all symptomatic plants and but not from salubrious sample ( Fig . 3 ) . The mathematical product was cloned and sequenced and the sequence deposited in GenBank ( Accession No . MF989441 ) . Analysis of the obtained succession revealed that it had 89.18 - 98.07 % nucleotide and 87.32 - 98.91 % amino group Elvis identity with the N gene of GBNV isolates wedge in GenBank from different crop and regions . The highest identity was observed with a GBNV tomato isolate from Coimbatore ( AY472081 ) . Phylogenetic psychoanalysis by the neighbour - joining method acting using MEGA 7 software indicated that it grouped with other GBNV isolates ( Fig . 4 ) .
Figure 3 : Agarose colloidal gel analysis of RT - PCR mathematical product hyperbolize using serogroup IV tospovirus - specific reverse and Groundnut bud sphacelus computer virus - specific advancing primers .
flesh 4 : Phylogenetic analysis of N gene chronological succession of Groundnut bud necrosis virus ( GBNV ) on anthurium with other isolates of GBNV and Tomato spotted wilting virus .
The occurrence of member of the genusOrthotospovirus , such asTomato spotted wilt virusandImpatiens necrotic spot virus , which are transmitted by several metal money of thrips , on anthurium has been reported from different parts of the existence ( Uchidaet al . , 1999 , Ghotbi & Shahraeen , 2012 ) . In India , GBNV is the most widely circulate member of the genusOrthotospovirusand infects a wide sort of plant species ( Mandalet al . , 2012 ) . However , to the expert of our knowledge , anthurium is a new host for GBNV , not only in India but also worldwide . The severity of symptom observed on tailflower paint a picture that , GBNV is an important and emerging virus on this decorative plant life with the potential to get economical damage and therefore merit care .
author : BSPP New Disease Reports ( B.S. Amruta , V. LaxmiDevi , G.K. Ramegowda , G.K. Seetharamu , T.R. Usharani and M. Krishnareddy )
